Phospho-foxo1
WebPhospho-FoxO1 (Ser256) (E1F7T) recognizes endogenous levels of FoxO1 protein only when phosphorylated at Ser256. The antibody cross-reacts with overexpressed FoxO4 phosphorylated at Ser193 and may cross-react with overexpressed FoxO3a phosphorylated at Ser253. The antibody also cross-reacts with a protein of unknown origin around 160kD. WebFOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein.
Phospho-foxo1
Did you know?
WebApr 13, 2024 · Treatment of cancer cells with PI3K inhibitors or taxane causes FOXO1 localization in the nucleus, increased expression of pERK1/2, and drug resistance. These effects are reversed by administering a small FOXO1-derived phospho-mimicking peptide inhibitor in vitro and in mice. Our results show a tumor suppressor role of AKT … WebThe Phospho-FoxO1 (Ser251) assay measures FoxO1 when phosphorylated at Ser251. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis or transfer. The Phospho-FoxO1 (Ser251) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor.
WebApr 7, 2024 · FoxO1 was phosphorylated in hearts of wild-type mice with physiological cardiac hypertrophy induced by 3 wk of swim training, but not in transgenic mice with reduced PI3K (p110α) activity due to expression of a dominant negative mutant of PI3K ( 5 ). WebThe Phospho-FoxO1 (Ser251) assay measures FoxO1 when phosphorylated at Ser251. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, …
WebPhospho-FoxO1 (Ser256) UniProt ID: Q12778: NCBI: 2308: Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Description: FoxO1 (phospho-Ser256) antibody (FITC) Alternative names: WebJan 13, 2024 · Non-Akt phosphorylated FoxO1/3 can be transported into the nucleus to activate transcription, whereas Akt-phosphorylated FoxOs contained in the nucleus of quiescent cells can be exported by the carrier protein 14-3-3 and degraded by the ubiquitin-proteosomal pathway 20 – 22.
WebApr 5, 2024 · The rabbit anti-AMPK antibody (D5A2), rabbit anti-SIRT1 antibody (D1D7), rabbit anti-Phospho-FoxO1 (Ser256), and rabbit anti-Phospho-AMPK (Thr172) were purchased from Cell Signaling Technology (Danvers, MA, USA). The RIPA lysate was purchased from Kangwei Century Biotechnology (Cwbio, Beijing, China). The Tunel assay …
WebPhospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody detects exogenous levels of FoxO1 only when phosphorylated at serine 319 and exogenous levels of FoxO4 only when phosphorylated at serine 262. The antibody does not cross-react with FoxO1 phosphorylated at other sites, FoxO4 phosphorylated at other sites nor with FoxO3a phosphorylated at any … bliss club mahindra holidaysWebAnti-FOXO1A (phospho S256) antibody See all FOXO1A primary antibodies Description Rabbit polyclonal to FOXO1A (phospho S256) Host species Rabbit Tested applications … blissclub store mumbaiWebJan 25, 2024 · Total protein lysates were analyzed by western blot with anti-phospho-FOXO1 (phospho-Thr24, phospho-Ser256 and phospho-Ser319), anti-FOXO1 and anti-β-actin antibodies. (B) HEK293T cells were ... free 123movies video downloader onlineWebMar 28, 2024 · Rbl2 has emerged as an endogenous CDK2 inhibitor in some contexts. 15, 16 To determine whether Rbl2 inhibits cardiac CDK2 activity, neonatal rat cardiomyocytes (NRCMs) were transfected with Rbl2 small interfering RNA or a nontargeting control. As shown in Figure 1A, knockdown of Rbl2 significantly increased the protein level of … bliss club leggings reviewWebAug 2, 2005 · All reaction products were analyzed by Western blotting using anti-phospho-Foxo1 antibody or silver stain. Discussion. Our present study provides two findings about the consequences of Foxo1 acetylation, the attenuation of DNA-binding ability and the enhancement of the level of Foxo1 phosphorylation. We further explored the relevance of … free 123 xmas greeting cardsWebMar 27, 2024 · (A) Top: Representative immunoblots of phospho (p)-FOXO1/3a (Thr24/Thr32) and total FOXO1 from NRVM treated with BSA or myristate 500 μM (M500) for 24 h, unstimulated or stimulated with insulin 10 nM for 15 min. β-TUBULIN was used as a loading control. Bottom: Quantification of p-FOXO1/FOXO1 (n = 4). bliss cochrane rd nwWebSep 6, 2024 · However, the host-derived regulatory network that controls mucus secretion and thereby changes gut microbiota has not been well studied. Here, we identify that … free 123greetings birthday cards