WebDec 15, 2024 · This small set of “true positives” precludes any meaningful analysis of what makes a paralog pair likely to be SL. Combinatorial CRISPR screens of paralog pairs, which have assayed hundreds of paralog pairs in pooled screens, may provide a more comprehensive set of true positives and negatives. WebDec 15, 2024 · Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q. Author links open overlay panel Jasper E. Neggers 1 2 8, ... We examine CRISPR-SpCas9 and RNA-interference loss-of-function screens to identify new therapeutic targets associated with …
In vivo CRISPR screening identifies BAZ2 chromatin remodelers …
WebAug 27, 2024 · Postdoctoral Researcher. University of Melbourne. Feb 2024 - Dec 20241 year 11 months. Melbourne, Victoria, Australia. Supervisor: Prof. Malcolm McConville. Project: Generation and characterization of null mutants in Leishmania mexicana using CRISPR/Cas9 to identify potential drug targets. WebThe threat of climate change on agriculture is real and scary. We expect more severe droughts, less predictable precipitation, and other abiotic stressors to become more commonpla reflecting telescope mirror
Addgene: Human Paralog Knockout Library (pgPEN)
WebJan 3, 2024 · Recent focus on paralog synthetic lethality in cancer cells offers an opportunity to evaluate different CRISPR/Cas multiplexing technologies and improve on the state of the art. Here we report a meta-analysis of CRISPR genetic interactions screens, identifying a candidate set of background-independent paralog synthetic lethals, and find … WebTo identify digenic dependencies, we developed a CRISPR paralog targeting library to investigate the viability effects of disrupting 3,284 genes, 5,065 paralog pairs and 815 paralog families. We identified that dual inactivation of DUSP4 and DUSP6 selectively impairs growth in NRAS and BRAF mutant cells through the hyperactivation of MAPK ... WebDec 1, 2024 · A CRISPR paralog targeting library profiling 815 paralog families across 11 cell lines identifies DUSP4 and DUSP6 as paralog pairs whose combined inactivation confers sensitivity to cells... reflecting telescope utilises