Hif1a antibody
WebRabbit HIF1A Rabbit pAb (A21742), validaed in IHC and tested in Human. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. Replacement to Abcam, Santa Cruz, Sigma and CST antibody. WebUnder hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular …
Hif1a antibody
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WebUnder normoxic conditions HIF-1 alpha has a short half-life. It is largely undetectable in cells or tissues grown under normoxic conditions. It is stabilized only at O 2 concentrations below 5% and upon stabilization under hypoxic conditions HIF-1 translocates to the nucleus. Therefore we recommend western blots using nuclear extracts and running Hypoxia … WebThis antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology ...
WebBethyl Laboratories Anti-HIF1-alpha Polyclonal, Catalog # A300-286A. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC) and … Web13 de mai. de 2024 · DNA was immunoprecipitated from the sonicated cell lysates using anti-HIF1A antibody (Abcam) and immunoprecipitates were recovered by addition of Salmon Sperm DNA/Protein A Agarose-50% Slurry.
WebHá 2 dias · Results showed that the HIF1A-As2 promoter region was significantly enriched in MYC antibody-bound complexes compared to IgG. H3K4me3 and H3K27ac were used as positive controls (Fig. 7H ). Web3 de out. de 2024 · After 3 washes, they were incubated with anti-Ki67 (1:25, Abcam, Cat #ab 1667), anti-CD31 antibody (1:25, BD Biosciences, Cat #550274) at 4° C. overnight and were then incubated with either Alexa 647-conjugated anti-rabbit IgG (Life Technology), or Alexa 594-conjugated anti-rabbit IgG or Alexa 488 conjugated anti-rat IgG at room …
WebSequence. Sequence: Please enter your sequence in the 5' to 3' direction. Sequences must be composed solely of the nucleotides A, C, G, T. Please convert U's to T's and remove …
WebWestern blot - HIF1α Rabbit pAb (A11945) Western blot analysis of extracts of HeLa cells, using HIF1α antibody (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. blowfish sherpa lined sneaker bootiesWebMonoclonal Antibody for studying HIF2-alpha. Cited in 67 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, HIF-2α (D9E3) Rabbit Monoclonal Antibody (CST #7096) is ready to ship. blowfish shoes women\u0027s blackWebHIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存，需避免反复冻融。 Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 blowfish shoes for women redblowfish shoes shoe carnivalWebHIF1a/HIF-1 alpha antibody used for IHC-Fr on frozen liver sections of pair- or ethanol-fed C57BL/6J wild-type mice (Figure 1B) and hepatocyte-specific HIF1a-deficint mice /delta HepHIF1a-/- on C57BL/6 background … blowfish shoe sizingWebMonoclonal Antibody for studying HIF1A. Cited in 31 publications. Validated for WB, IP, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, HIF-1α (D5F3M) … free express vpn accountsWebI am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. blowfish security