Grf–gif chimeric protein
WebNew opportunities for using WUS/BBM and GRF-GIF genes to enhance genetic transformation of ornamental plants New opportunities for using WUS / BBM and GRF-GIF genes to enhance genetic transformation of ornamental plants Hui Duan 1 , , , , Nathan A. Maren 2 , Thomas G. Ranney 3 & Wusheng Liu 2 , , , 1. We generated the Citrus and Vitis GRF–GIF chimeras by gene synthesis using the GRF and GIF homologs highlighted in Supplementary Fig. 1. We cloned the DNA fragments into pDONR by a Gateway BP reaction. We cloned the GRF–GIF chimeras into the pGWB14 binary vector by a Gateway LR reaction under … See more We performed all PCR cloning with Phusion High-Fidelity DNA Polymerase (New England BioLabs). We extracted RNA from spikes … See more Wheat transformation followed previously published protocols29. Briefly, we grew the different wheat and triticale cultivars in a greenhouse or a growth chamber under a long-day … See more We placed seeds of Carrizo citrange rootstock in water to soak and then peeled off the seed coats, making sure not to remove the … See more Rice transformation was performed following previously published protocols36. Briefly, we selected fresh rice seeds, dehusked them and … See more
Grf–gif chimeric protein
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WebA GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J Nat Biotechnol. 2024 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. PubMed Article Plasmids from Article. ID Plasmid Purpose ... WebGRF-GIF chimeric proteins from multiple source species enhance in vitro regeneration in both wild and cultivated lettuce. In addition, they enhance regeneration in multiple types …
WebA GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Nat Biotechnol. 2024 Nov;38 (11):1274-1279. doi: 10.1038/s41587-020-0703-0. Epub 2024 … WebJul 15, 2024 · GRF–GIF chimeric proteins enhance in vitro regeneration and Agrobacterium-mediated transformation efficiencies of lettuce (Lactuca spp.) 2024, Plant …
WebMar 1, 2024 · Low regeneration efficiency hampers plant transformation. Two independent studies demonstrate that GROWTH-REGULATING FACTORs (GRFs) alone or in chimeras with GRF-INTERACTING FACTOR (GIF) dramatically boost regeneration from tissue cultures from a broad range of species. GRF-GIF chimeras could be a game-changer in … WebNov 16, 2024 · GRF-GIF chimeric proteins from multiple source species enhance in vitro regeneration in wild and multiple types of cultivated lettuce, including butterheads, …
WebAug 24, 2024 · Here, we show that expression of a chimeric protein including wheat GROWTH-REGULATING FACTOR 4 (GRF4) and its cofactor GRF-INTERACTING FACTOR 1 (GIF1) dramatically increases the efficiency...
WebJan 14, 2024 · Furthermore, Growth-Regulating Factor 4 (GRF4) and its cofactor GRF-Interacting Factor 1 (GIF1) have been recently shown to increase the transformation frequencies in both monocots and dicots, most likely by regulating the cell proliferation and in the transition between stem cells to transit-amplifying cells. hennmars horrormarathonWeb3 52 The GRF proteins form complexes with GIF cofactors that also interact with chromatin remodeling 53 complexes in vivo 15, 16.Multiple levels of regulation control the efficiency of the assembly of 54 functional GRF/GIF complexes in vivo 17.Loss-of-function mutations in GIF genes mimic the 55 reduced organ size observed in GRF loss-of-function mutants or … lasko space heater model 5622 electricWebOct 12, 2024 · Here, we show that expression of a fusion protein combining wheat GROWTH-REGULATING FACTOR 4 (GRF4) and its cofactor GRF-INTERACTING … hennly picsWebDec 15, 2024 · A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants. Nat. Biotechnol. 38, 1274–1279 (2024). Article CAS Google Scholar ... hennman freight \u0026 logisticsWebOct 16, 2024 · Debernardi et al. designed a chimeric GRF/GIF fusion protein which they showed accelerates regeneration of green shoots from callus tissue and can do so without culturing on cytokinins. Interestingly, … hennlich romaniaWebA red fluorescent protein-encoding gene was added to both constructs to enable rapid quantification of transgenic regeneration rates. The chimeric GRF-GIF sequences were cloned into an expression vector and poplar as well as eucalypt tissue explants were transformed using Agrobacterium. lasko space heater wattageWebMar 1, 2024 · The GRF-GIF duo regulates the ATP-dependent DNA translocase SWI2/SNF2. (B) GIF-GRF chimeras boost plant regeneration. (Top) Plant tissues can be … lasko tower fan model 2551