WebFluorescein isothiocyanate–dextran (FITC-Dextran 70) is use as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study … WebName matches: dextran fitc; fluorescein isothiocyanate. Destruction of polyelectrolyte microcapsules and release of FITC-dextran from them by the influence of sodium …
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WebPBS buffer. Two types of anti-IgG, FITC-anti-human IgG (Sigma-Aldrich) and TRITC -anti-rabbit IgG (Sigma-Aldrich) were used. Microfibers were incubated with these three solutions for 30 minat 35 ℃ and washed with DI water for several times. A confocal laser scanning microscope (LSM 5 PASCAL, Web1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of 1 x 10^6 cells/ml. 2. Transfer 100 µl of the solution (1 x 10^5 cells) to a 5 ml culture tube. 3. Add 5 µl of FITC Annexin V and 5 µl PI. 4. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 5. red carpet pro led light
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